Terahertz time domain spectroscopy of amino acids and sugars Citation Kelley, Matthew James Terahertz time domain spectroscopy of amino acids and sugars. Due to there being few existing methods of generating and detecting THz radiation, the spectrometer is expected to have vast applications to solid, liquid, and gas phase samples.
Studies on amino acid activation and protein synthesis Citation Clark, John Magruder Studies on amino acid activation and protein synthesis. Several different experimental approaches to the problem are described. The initial studies concern the incorporation of amino acids into tissue sections and homogenates.
Tissue sections show a constant rate of incorporation of labeled amino acids into protein for periods of time up to two hours. The rate of incorporation then decreases with time. Conversely, tissue homogenates show ever increasing rates of incorporation of labeled amino acids into protein.
This incorporation is indicative of incorporation of labeled amino acids into bacteria within the homogenate rather than incorporation into plant protein. Sterile homogenate preparations do not show the kinetics of incorporation shown by other homogenate preparations.
The incorporation of labeled amino acids into acid insoluble material obtained from sterile tissue homogenates is a function of the washing procedure used in the assay. Amino acid activation has been studied by a hydroxylamine trapping reaction and by pyrophosphate exchange. Plant tissues contain amino acid activating enzymes which may be detected by either method of assay.
The pyrophosphate exchange assay is however more sensitive. The hydroxamate assay suffers from some added complications common to plant systems. The specific nature of amino acid activation in plant preparations has been studied with enzymes isolated from spinach leaves.
The enzymes occur in all plant tissues tested and appear to be a part of the soluble fraction of cells. Purification procedures have been developed which make it possible to remove the free amino acids from the preparations. The purified preparation is capable of activating all of the naturally occurring in protein amino acids except serine.
The possible activation of serine is-not excluded. Preliminary evidence is presented which indicates that each amino acid is activated by its own specific amino acid activating enzyme.
The possibility of linking the process of amino acid activation to protein synthesis is considered. Even so it has been possible to demonstrate an ATP dependent RNAase sensitive incorporation of labeled leucine into acid and alcohol washed material.lausannecongress2018.com Page CHAPTER 24 Amino Acids, Peptides, and Proteins amino acids, called the essential amino acids, must be provided in the diet.
The ten thesis of phenylalanine.
Deprived of phenylalanine, the plant cannot make the proteins it. INTRODUCTION Derivatization of amino acids by dansylation is commonly used to identify N-terminal amino acids of peptides and constituent amino acids of protein hydrolysates. tial amino acids (EAAs), meaning they cannot be pro-duced by the body in physiologically significant amounts, and therefore are crucial components of a balanced diet.
thesis cannot be sustained, there is little physiological sig . Amino acid synthesis is the set of biochemical processes (metabolic pathways) by which the various amino acids are produced from other compounds. The substrates for these processes are various compounds in the organism 's diet or growth media.
A THESIS SUBMITTED TO THE GRADUATE DIVISION OF THE UNIVERSITY OF HAWAI’I AT MANOA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF Some amino acids, known as “trophic” amino acids (alanine, aspartic acid, glutamic acid, isoleucine, proline, valine), fractionate in a predictable way (~ Biochem: Protein and Amino Acids Essay examples.
chemical elements in living things are carbon, hydrogen, oxygen and nitrogen (HONC) State that a variety of other elements are needed by living organisms, including sulfur, calcium, phosphorus, iron and sodium.